Create/Define realiquoted aliquots

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Contents

Introduction

This guide will describe the process to define aliquots as created from other aliquots of the same sample. 

This process can be used to: 
  1- Create 2 tubes of a DNA solution from one parent before shipping.
  2- Create one tissue slide from a tissue block.
  3- Create a tissue core from a tissue block.
 
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Realiquote Tubes

This section will detail the data entry steps that allow user to define 2 DNA tubes as created from one parent DNA tube.


1. Open the DNA extraction 'Details' form. Click on 'Add Aliquot' to create the 2 realiquoted tubes created from the parent DNA tube.

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2. See "Create collected specimens and aliquots" guide to create DNA tubes.

3. Click on the 'Detail' icon of the DNA parent aliquot.

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4. In the 'Details' form of the parent aliquot, click on 'Define Realiquoted Children' button.

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5. Select the 2 aliquots previously created and that should be defined as realiquoted aliquots.

Notes: User can define the parent aliquot volume used to create each realiquoted tube.

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6. Complete additional information and click submit.

7. Into the 'Details' form of the parent aliquot, the current volume of this aliquot will be updated and the realiquoting information will be displayed into the 'Uses' section.

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8. Into the 'Details' form of the DNA sample, all aliquots will be listed plus their relationship ('Parent'/'Child').

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9. For each realiquoted tube, the realiquoting information can be display clicking on the 'Realqiuoted Parent' menu of the aliquot 'Details' form.

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Create tissue slide from tissue block

This section will detail the data entry steps that allow user to define 1 tissue slide as created from one tissue block.

Note: This step is not detailed in deep. To have more details, please see the previous section.


1. Open the Tissue sample 'Details' form. Click on 'Add Aliquot' to create the tissue slide.

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2. Create the slide.

3. Open the 'Details' form of the tissue block and click on 'Define Realiquoted Children' button.

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4. Select the slide previously created and that should be defined as block slide.

Realiquote13.jpg

6. Complete additional information and click submit.

Conclusions

This process could be reproduced to create any parent/child relationship between 2 aliquots of a same sample: 
  - Create a tissue core from a tissue block.
  - Create a tissue block from a tissue tube.
  - Create a whatman paper from a blood tube.
  - Etc.
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